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ponceau s total protein stain  (Millipore)


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    Structured Review

    Millipore ponceau s total protein stain
    Ponceau S Total Protein Stain, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ponceau+s+total+protein+staining/ponceau+s+total+protein+stain/pm39032600-112-10-7
    Average 90 stars, based on 1 article reviews
    ponceau s total protein stain - by Bioz Stars, 2026-11
    90/100 stars

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    Related Articles

    Membrane:

    Article Title: Probenecid Disrupts a Novel Pannexin 1-Collapsin Response Mediator Protein 2 Interaction and Increases Microtubule Stability.
    Article Snippet: .. Gels were transferred to 0.2 μm polyvinyldene fluoride (PVDF; Bio-rad, 162-0177) membrane for 1 h at 85–95 V, or 16–18 h at 22 V. Transfer was confirmed by Ponceau S (MilliporeSigma, P7170) total protein staining. .. Blocking and antibody incubations were performed in 5% skim milk, or 5% bovine serum albumin (BSA; MilliporeSigma, A2153), both with 0.1% Tween 20.

    Article Title: Fetal sex and the relative reactivity of human umbilical vein and arteries are key determinants in potential beneficial effects of phosphodiesterase inhibitors
    Article Snippet: .. A Ponceau S total protein staining (Sigma-Aldrich, cat. no. P7170) was performed to visualize proteins and the stained membrane was scanned, using an office scanner, to allow further normalization. .. A Ponceau S total protein staining (Sigma-Aldrich, cat. no. P7170) was performed to visualize proteins and the stained membrane was scanned, using an office scanner, to allow further normalization.

    Article Title: Fetal sex and the relative reactivity of human umbilical vein and arteries are key determinants in potential beneficial effects of phosphodiesterase inhibitors.
    Article Snippet: Intrauterine growth restriction (IUGR) is a common complication of pregnancy.. We previously demonstrated that IUGR is associated with an impaired nitric oxide (NO)-induced relaxation in the human umbilical vein (HUV) of growth-restricted females compared to appropriate for gestational age (AGA) newborns.. We found that phosphodiesterase (PDE) inhibition improved NOinduced relaxation in HUV, suggesting that PDEs could represent promising targets for therapeutic intervention.

    Article Title: Probenecid Disrupts a Novel Pannexin 1-Collapsin Response Mediator Protein 2 Interaction and Increases Microtubule Stability
    Article Snippet: .. Gels were transferred to 0.2 μm polyvinyldene fluoride (PVDF; Bio-rad, 162-0177) membrane for 1 h at 85–95 V, or 16–18 h at 22 V. Transfer was confirmed by Ponceau S (MilliporeSigma, P7170) total protein staining. .. Blocking and antibody incubations were performed in 5% skim milk, or 5% bovine serum albumin (BSA; MilliporeSigma, A2153), both with 0.1% Tween 20.

    Article Title: DJ-1 interacts with the ectopic ATP-synthase in endothelial cells during acute ischemia and reperfusion
    Article Snippet: 10 μg of total protein were loaded and separated by SDS-PAGE with a Mini protean 3 system (Bio-Rad Laboratories, Hercules, CA, USA). .. Proteins were then transferred to a nitrocellulose membrane (1620115; Bio-Rad Laboratories, Hercules, CA, USA), and immediately stained with Ponceau S total protein staining (P3504; Sigma, Saint Louis, MI, USA), to normalize differences in the loaded protein amounts between lanes. .. Blots were then blocked with 5 % bovine serum albumin (MB04603; NZYTech, Lisboa, Portugal) diluted in TBS-Tween20 and incubated overnight with either mouse anti-DJ-1 (MCA-4H4, EnCor Biotechnology, Gainesville, FL, USA) at 1:4000, rabbit anti-ATP5B (MA5-32589, Invitrogen, Carlsbad, CA, USA) at 1:1000, rabbit anti-phospho-Akt Ser473 (4060; Cell Signaling Technology, Danvers, MA, USA) at 1:1000, rabbit anti-Akt (9272; Cell Signaling Technology, Danvers, MA, USA) at 1:1000, or rabbit anti-Casp-3 (9662; Cell Signaling Technology, Danvers, MA, USA) at 1:1000.

    Staining:

    Article Title: Probenecid Disrupts a Novel Pannexin 1-Collapsin Response Mediator Protein 2 Interaction and Increases Microtubule Stability.
    Article Snippet: .. Gels were transferred to 0.2 μm polyvinyldene fluoride (PVDF; Bio-rad, 162-0177) membrane for 1 h at 85–95 V, or 16–18 h at 22 V. Transfer was confirmed by Ponceau S (MilliporeSigma, P7170) total protein staining. .. Blocking and antibody incubations were performed in 5% skim milk, or 5% bovine serum albumin (BSA; MilliporeSigma, A2153), both with 0.1% Tween 20.

    Article Title: Fetal sex and the relative reactivity of human umbilical vein and arteries are key determinants in potential beneficial effects of phosphodiesterase inhibitors
    Article Snippet: .. A Ponceau S total protein staining (Sigma-Aldrich, cat. no. P7170) was performed to visualize proteins and the stained membrane was scanned, using an office scanner, to allow further normalization. .. A Ponceau S total protein staining (Sigma-Aldrich, cat. no. P7170) was performed to visualize proteins and the stained membrane was scanned, using an office scanner, to allow further normalization.

    Article Title: Fetal sex and the relative reactivity of human umbilical vein and arteries are key determinants in potential beneficial effects of phosphodiesterase inhibitors.
    Article Snippet: Intrauterine growth restriction (IUGR) is a common complication of pregnancy.. We previously demonstrated that IUGR is associated with an impaired nitric oxide (NO)-induced relaxation in the human umbilical vein (HUV) of growth-restricted females compared to appropriate for gestational age (AGA) newborns.. We found that phosphodiesterase (PDE) inhibition improved NOinduced relaxation in HUV, suggesting that PDEs could represent promising targets for therapeutic intervention.

    Article Title: Probenecid Disrupts a Novel Pannexin 1-Collapsin Response Mediator Protein 2 Interaction and Increases Microtubule Stability
    Article Snippet: .. Gels were transferred to 0.2 μm polyvinyldene fluoride (PVDF; Bio-rad, 162-0177) membrane for 1 h at 85–95 V, or 16–18 h at 22 V. Transfer was confirmed by Ponceau S (MilliporeSigma, P7170) total protein staining. .. Blocking and antibody incubations were performed in 5% skim milk, or 5% bovine serum albumin (BSA; MilliporeSigma, A2153), both with 0.1% Tween 20.

    Article Title: DJ-1 interacts with the ectopic ATP-synthase in endothelial cells during acute ischemia and reperfusion
    Article Snippet: 10 μg of total protein were loaded and separated by SDS-PAGE with a Mini protean 3 system (Bio-Rad Laboratories, Hercules, CA, USA). .. Proteins were then transferred to a nitrocellulose membrane (1620115; Bio-Rad Laboratories, Hercules, CA, USA), and immediately stained with Ponceau S total protein staining (P3504; Sigma, Saint Louis, MI, USA), to normalize differences in the loaded protein amounts between lanes. .. Blots were then blocked with 5 % bovine serum albumin (MB04603; NZYTech, Lisboa, Portugal) diluted in TBS-Tween20 and incubated overnight with either mouse anti-DJ-1 (MCA-4H4, EnCor Biotechnology, Gainesville, FL, USA) at 1:4000, rabbit anti-ATP5B (MA5-32589, Invitrogen, Carlsbad, CA, USA) at 1:1000, rabbit anti-phospho-Akt Ser473 (4060; Cell Signaling Technology, Danvers, MA, USA) at 1:1000, rabbit anti-Akt (9272; Cell Signaling Technology, Danvers, MA, USA) at 1:1000, or rabbit anti-Casp-3 (9662; Cell Signaling Technology, Danvers, MA, USA) at 1:1000.



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